Open AccessIn situ aneuploidy assessment in human sperm: the use of primed in situ and peptide nucleic acid‐fluorescence in situ hybridization techniques
Author(s) -
Pellestor Franck
Publication year - 2006
Publication title -
asian journal of andrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 74
eISSN - 1745-7262
pISSN - 1008-682X
DOI - 10.1111/j.1745-7262.2006.00137.x
Subject(s) - fluorescence in situ hybridization , in situ , in situ hybridization , peptide nucleic acid , aneuploidy , nucleic acid , biology , microbiology and biotechnology , primer (cosmetics) , molecular beacon , hybridization probe , dna , biochemistry , chemistry , oligonucleotide , messenger rna , gene , chromosome , organic chemistry
Both the primed in situ (PRINS) and the peptide nucleic acid‐fluorescence in situ hybridization (PNA‐FISH) techniques constitute alternatives to the conventional (fluorescence in situ hybridization, FISH) procedure for chromosomal investigations. The PRINS reaction is based on the use of a DNA polymerase and labeled nucleotide in an in situ primer extension reaction. Peptide nucleic acid probes are synthetic DNA analogs with uncharged polyamide backbones. The two procedures present several advantages (specificity, rapidity and discriminating ability) that make them very attractive for cytogenetic purposes. Their adaptation to human spermatozoa has allowed the development of new and fast procedures for the chromosomal screening of male gametes and has provided efficient complements to FISH for in situ assessment of aneuploidy in male gametes.