Open AccessDAZ1/DAZ2 cluster deletion mediated by gr/gr recombination per se may not be sufficient for spermatogenesis impairment: a study of Chinese normozoospermic men
Author(s) -
Yang Yuan,
Xiao CuiYing,
A ZhouCun,
Zhang SiZhong,
Li Xiang,
Zhang SiXiao
Publication year - 2006
Publication title -
asian journal of andrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 74
eISSN - 1745-7262
pISSN - 1008-682X
DOI - 10.1111/j.1745-7262.2006.00121.x
Subject(s) - azoospermia , genetics , azoospermia factor , biology , spermatogenesis , polymerase chain reaction , microbiology and biotechnology , population , single nucleotide polymorphism , andrology , gene , genotype , medicine , infertility , endocrinology , pregnancy , environmental health
Aim: To explore the possible effect of the deleted in azoospermia ( DAZ ) copy cluster deletion on spermatogenesis in the Chinese population, the deletion of the azoospermia factor c ( AZFc ) region was analyzed in 346 normozoospermic men. Methods: Three DAZ single nucleotide variant loci and seven AZFc‐specific sequence‐tagged sites were examined with polymerase chain reaction (PCR)‐restriction fragment length polymorphism and routine PCR. Results: Five (1.4%) of the normozoospermic men were found to have deletion of gr/gr‐DAZ1/DAZ2. None of the men were found to have b2/b4‐entire DAZ deletion. Conclusion: The presence of gr/gr‐DAZ1 /DAZ2 deletion in five men with normozoospermia suggests that this deletion per se may not be sufficient for spermatogenic impairment in Chinese men.