Open AccessExpression of a novel dipeptidyl peptidase 8 ( DPP8 ) transcript variant, DPP8‐v3 , in human testis
Author(s) -
Zhu Hui,
Zhou ZuoMin,
Lu Li,
Xu Min,
Wang Hui,
Li JianMin,
Sha JiaHao
Publication year - 2005
Publication title -
asian journal of andrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 74
eISSN - 1745-7262
pISSN - 1008-682X
DOI - 10.1111/j.1745-7262.2005.00054.x
Subject(s) - biology , complementary dna , dipeptidyl peptidase , reverse transcription polymerase chain reaction , dipeptidyl peptidase 4 , messenger rna , endocrinology , gene expression , medicine , gene , microbiology and biotechnology , enzyme , genetics , biochemistry , diabetes mellitus , type 2 diabetes
Aim: To investigate the role of a novel dipeptidyl peptidase 8 transcript variant ( DPP8‐v3 ) gene in testis development and/or spermatogenesis. Methods :A human testis cDNA microarray was hybridized with mRNA of human adult and fetal testes. Differentially expressed clones were sequenced and characterized and their expression was analyzed by real‐time reverse transcription polymerase chain reaction (RT‐PCR) and Southern‐blot analysis. Results :A new transcript variant of the human dipeptidyl peptidase ( DPP8 ), exhibiting a 5‐fold higher expression level in human adult than that in fetal testes, was cloned and was named DPP8 variant 3 ( DPP8‐v3 ).The full‐length sequence of DPP8‐v3 was 3,030 bp, encoding a protein of 898 amino acids. Conclusion : DPP8‐v3 is a novel human DPP8 transcript variant highly expressed in the adult testis. Similar to DPPIV, DPP8‐v3 may play a key role in the immunoregulation of testes and accordingly may influence spermatogenesis and male fertility.