Open AccessSuberoyl bis ‐hydroxamic acid induces p53‐dependent apoptosis of MCF‐7 breast cancer cells 1
Author(s) -
ZHUANG Zhigang,
FEI Fei,
CHEN Ying,
JIN Wei
Publication year - 2008
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2008.00906.x
Subject(s) - apoptosis , mcf 7 , puma , microbiology and biotechnology , flow cytometry , blot , cancer cell , chemistry , cancer research , histone deacetylase inhibitor , biology , histone , dna , cancer , histone deacetylase , biochemistry , gene , human breast , genetics
Aim: To study the effects of suberoyl bis‐hydroxamic acid (SBHA), an inhibitor of histone deacetylases, on the apoptosis of MCF‐7 breast cancer cells. Methods: Apoptosis in MCF‐7 cells induced by SBHA was demonstrated by flow cytometric analysis, morphological observation, and DNA ladder. Mitochondrial membrane potential (ΔΨm) was measured using the fluorescent probe JC‐1. The expressions of p53, p21, Bax, and PUMA were determined using RT‐PCR or Western blotting analysis after the MCF‐7 cells were treated with SBHA or p53 siRNA. Results: SBHA induced apoptosis in MCF‐7 cells. The expressions of p53, p21, Bax, and PUMA were induced, and ΔΨm collapsed after treatment with SBHA. p53 siRNA abrogated the SBHA‐induced apoptosis and the expressions of p53, p21, Bax, and PUMA. Conclusion: The activation of the p53 pathway is involved in SBHA‐induced apoptosis in MCF‐7 cells.