Open AccessAspergillus fumigatus conidia upregulates NOD2 protein expression both in vitro and in vivo 1
Author(s) -
ZHANG Huijun,
QU Jieming,
SHAO Changzhou,
ZHANG Jing,
HE Lixian,
YUAN Zhenghong
Publication year - 2008
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2008.00860.x
Subject(s) - aspergillus fumigatus , nod2 , conidium , microbiology and biotechnology , a549 cell , tumor necrosis factor alpha , biology , western blot , in vivo , in vitro , immunology , immune system , biochemistry , innate immune system , botany , gene
Aim: To determine if NOD2 is involved in host recognition of Aspergillus fumigatus (Af) conidia. Methods: An Af conidia pulmonary infection murine model was established by intranasal inoculation of Af onidia suspensions. Protein levels of NOD2 in lung tissue were determined by immunohistochemistry. A549 and phorbol‐12‐myristate 13‐acetate (PMA)‐activated THP‐1 cell lines were treated with heat‐killed Af conidia, then the presence of NOD2 protein in these cell lines was detected by Western blotting. The ability of muramyl dipeptide (MDP) to induce the secretion of TNF‐α after incubation with heat‐killed Af conidia was measured by enzyme‐linked immunosorbent assay. Results: The expression of NOD2 protein in lung tissue increased after Af conidia infection. Heat‐killed Af conidia significantly upregulated NOD2 protein expression in A549 cells and PMA‐activated THP‐1 cells. Additionally, Af conidia in conjuction with MDP, significantly increased the secretion of TNF‐α in A549 cells and PMA‐activated THP‐1 cells. Conclusion: Af conidia upregulates NOD2 protein expression in vitro and in vivo . These findings indicate that NOD2 protein may respond to Af conidia.