Open AccessGinkgo biloba extract prevents against apoptosis induced by high glucose in human lens epithelial cells 1
Author(s) -
WU Zhengmei,
YIN Xiaoxing,
JI Lei,
GAO Yuanyuan,
PAN Yingmei,
LU Qian,
WANG Jianyun
Publication year - 2008
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2008.00837.x
Subject(s) - apoptosis , ginkgo biloba , oxidative stress , caspase 3 , pharmacology , chemistry , tunel assay , viability assay , aldose reductase , programmed cell death , biochemistry , biology , enzyme
Aim: To investigate the protective effects of Ginkgo biloba extract (GEE) on high glucose‐induced apoptosis of human lens epithelial cells (HLEC) and the possible molecular mechanisms. Methods: The cultured HLEC were allotted into 6 groups: normal group, high glucose group, low‐, moderate‐, and high‐dose GB E group, and the bendazac lysine group. Cell viability, cell apoptosis, the activities of cell antioxidases, aldose reductase, caspase‐3, the levels of cell antioxidants, and the expressions of Bcl‐2 and Bax were assessed by different methods. Results: After being incubated with high glucose for 24 h, HLEC underwent apoptosis and exhibited significant oxidative stress. In the presence of GB E at different doses, the rate of HLEC apoptosis was lower and the oxidative stress state was significantly ameliorated. The increased ratio of Bax to Bcl‐2 was significantly reduced and the activation of caspase‐3 was suppressed by GB E in a dose‐dependent manner. Conclusion: GB E prevents HLEC from high glucose‐induced apoptosis through inhibiting oxidative stress, reducing the ratio of Bax to Bcl‐2, and decreasing the activity of caspase‐3. Therefore, GB E has a potential protective effect against diabetic cataract formation.