Open AccessLY294002 induces p53‐dependent apoptosis of SGC7901 gastric cancer cells 1
Author(s) -
XING Chungen,
ZHU Baosong,
LIU Huihui,
LIN Fang,
YAO Huihua,
LIANG Zhongqin,
QIN Zhenghong
Publication year - 2008
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2008.00770.x
Subject(s) - apoptosis , propidium iodide , flow cytometry , viability assay , microbiology and biotechnology , mtt assay , cell cycle , biology , chemistry , ly294002 , pi3k/akt/mtor pathway , programmed cell death , biochemistry
Aim: To study the effects of LY294002, an inhibitor of class I phosphatidylinositol 3‐kinase (PI3K), on proliferation and apoptosis of SGC7901 gastric cancer cells. Methods: The MTT assay was used to determine the cytotoxic effects of LY294002. Cell cycle distribution was analyzed using flow cytometry and apoptosis was assessed using flow cytometry analysis after staining DNA with propidium iodide. Mitochondrial membrane potential was measured using the fluorescent probe JC‐1. Expression of p53 and PUMA was determined using real‐time RT‐PCR and Western blotting analysis. Results: The viability of SGC7901 cells was significantly reduced by LY294002 treatment. Expression of p53 and PUMA was induced, and mitochondrial membrane potential collapsed after treatment with LY294002. LY294002 induced apoptotic cell death. Conclusion: Activation of the p53 pathway is involved in LY294002‐induced SGC7901 cell death.