Open AccessThe interaction of small molecules with phospholipid membranes studied by 1 H NOESY NMR under magic‐angle spinning 1
Author(s) -
SCHEIDT Holger A,
HUSTER Daniel
Publication year - 2008
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2008.00726.x
Subject(s) - membrane , magic angle spinning , two dimensional nuclear magnetic resonance spectroscopy , chemistry , lipid bilayer , molecule , phospholipid , intermolecular force , proton nmr , solid state nuclear magnetic resonance , nuclear magnetic resonance spectroscopy , crystallography , stereochemistry , nuclear magnetic resonance , organic chemistry , biochemistry , physics
The interaction of small molecules with lipid membranes and the exact knowledge of their binding site and bilayer distribution is of great pharmacological importance and represents an active field of current biophysical research. Over the last decade, a highly resolved 1 H solid‐state NMR method has been developed that allows measuring localization and distribution of small molecules in membranes. The classical solution 1 H NMR NOESY technique is applied to lipid membrane samples under magic‐angle spinning (MAS) and NOESY cross‐relaxation rates are determined quantitatively. These rates are proportional to the contact probability between molecular segments and therefore an ideal tool to study intermolecular interactions in membranes. Here, we review recent 1 H MAS NOESY applications that were carried out to study lateral lipid organization in mixed membranes and the interaction of membranes with water, ethanol, small aromatic compounds, peptides, fluorescence labels, and lipophilic nucleosides.