Open AccessAntioxidant properties of berberine on cultured rabbit corpus cavernosum smooth muscle cells injured by hydrogen peroxide 1
Author(s) -
TAN Yan,
TANG Qiang,
HU Benrong,
XIANG Jizhou
Publication year - 2007
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2007.00705.x
Subject(s) - berberine , hydrogen peroxide , antioxidant , smooth muscle , rabbit (cipher) , pharmacology , chemistry , anatomy , medicine , microbiology and biotechnology , biology , endocrinology , biochemistry , statistics , mathematics
Aim : To investigate the antioxidant properties of berberine (Ber) on corpus cavenosum smooth muscle cells (CCSMC) in penile erectile dysfunction. Methods : We examined the effects of Ber on cultured rabbit CCSMC damaged by hydrogen peroxide (H 2 O 2 ) through examining cell viability by methyl thiazolyl tetrazolium assay and assessing the level of malondialdehyde (MDA), superoxide dismutase (SOD) activity, nitric oxide (NO) products, and lactate dehydrogenase (LDH) release in cells after stimulation with H 2 O 2 . Results : Treatment with 1 mmol/L H 2 O 2 significantly decreased the cell viability, NO products, and SOD activity of CCSMC from 100% to 48.57%±4.1% ( P <0.01), 66.8±16.3 to 6.7±2.1 μmol/L ( P <0.01), and 49.5±1.8 to 30.1±2.6 U/mL ( P <0.01), respectively, and increased LDH release and MDA content from 497.6±69.5 to 1100.5±56.3 U/L ( P <0.01) and 3.7±1.3 to 78.4±2.9 nmol/mg protein ( P <0.01), respectively. However, treatment with different concentrations of Ber (10‐1000 μmol/L) inhibited the damaging effects of H 2 O 2 , with increased cell viability ( P <0.05 or P <0.01), NO production ( P <0.01), and SOD activity ( P <0.01) and decreased LDH release and MDA content (both P <0.01). Conclusion : Ber could produce its antioxidant action on oxidative stress‐induced cultured CCSMC. These effects may be of benefit in the prevention of penile erectile dysfunction.