Open Access11 beta‐hydroxysteroid dehydrogenase type 1 promotes differentiation of 3T3‐L1 preadipocyte 1
Author(s) -
LIU Yun,
SUN Yan,
ZHU Ting,
XIE Yu,
YU Jing,
SUN Wenlan,
DING Guoxian,
HU Gang
Publication year - 2007
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2007.00602.x
Subject(s) - transfection , 3t3 l1 , cellular differentiation , adipocyte , adipogenesis , biology , cell culture , lipoprotein lipase , endocrinology , medicine , microbiology and biotechnology , adipose tissue , gene , biochemistry , genetics
Aim: To investigate the relationship between 11 beta‐hydroxysteroid dehydrogenase type 1 (11 beta‐HSD1), a potential link between obesity and type 2 diabetes, and preadipocyte differentiation. Methods: Mouse 11 beta‐HSD1 siRNA plasmids were transfected into 3T3‐L1 preadipocytes (a cell line derived from mouse Swiss3T3 cells that were isolated from mouse embryo), for examination of the effect of targeted 11 beta‐HSD1 inhibition on differentiation of 3T3‐L1 cells. Differentiation was stimulated with 3‐isobutyl‐1‐methyxanthine, insulin, and dexamethasone. The transcription level of the genes was detected by real‐time PCR. Results: Lipid accumulation was significantly inhibited in cells transfected with mouse 11 beta‐HSD1 siRNA compared with non‐transfected 3T3‐L1 cells. Fewer lipid droplets were detected in the transfected cells both prior to stimulation and after stimulation with differentiation‐inducing reagents. The expression of adipocyte differentiation‐associated markers such as lipoprotein lipase and fatty acid synthetase were downregulated in the transfected cells. Similarly, the expression of preadipocyte factor‐1, an inhibitor of adipocyte differentiation, was downregulated upon stimulation of differentiation and had no changes in the transfected cells. Conclusion: 11 beta‐HSD1 can promote preadipocyte differentiation. Based on this, we propose that 11 beta‐HSD1 may be an important candidate mediator of obesity and obesity‐induced insulin resistance.