Open AccessProtective effects of heat shock protein70 induced by geranylgeranylacetone in atrophic gastritis in rats 1
Author(s) -
LIU Weili,
CHEN Shujie,
CHEN Yan,
SUN Leimin,
ZHANG Wei,
ZENG Yamin,
ZHOU Tianhua,
SI Jianmin
Publication year - 2007
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2007.00589.x
Subject(s) - atrophic gastritis , gastritis , hsp70 , medicine , gastroenterology , inflammation , gastric mucosa , antrum , immunohistochemistry , pathological , pathology , lamina propria , stomach , heat shock protein , chemistry , epithelium , biochemistry , gene
Aim : To investigate the effect of geranylgeranylacetone (GGA) on the progression of atrophic gastritis in rats and its potential mechanism. Methods : Atrophic gastritis was induced in Sprague‐Dawley rats with 0.1% ammonia solution, 60% ethanol, and 20 mmol/L deoxycholic acid for 24 weeks. Accompanied by the induction of atrophic gastritis, 200 mg/kg GGA was administered by oral gavage for 8 weeks (weeks 17‐24). The histological changes in gastric mucosa were quantitated by the index of inflammation, the gastric mucosal thickness, and the amount of glands of 1 mm horizontal length in antrum. Endogenous heat shock protein (HSP)70 levels and distribution were determined by immunoblotting and immunohistochemistry in gastric mucosa. Results : GGA alleviated the pathological progression of atrophic gastritis with inflammation relief (inflammation index: 1.40 in the GGA group and 1.65 in the atrophic gastritis group) and glandular restoration (mucosal thickness and quantity of glands: 194.3 μm and 38.7 mm in the GGA group; 123.3 μm and 32.7 mm in the atrophic gastritis group; P <0.05). GGA significantly induced HSP70 synthesis ( P <0.05). Moreover, quercetin, an inhibitor of HSP70 expression, aggravated the infiltration of inflammatory cells and glandular loss in the antrum. Conclusion : GGA prevented the progression of atrophic gastritis in rats via the induction of HSP70 expression.