Open AccessCysteinyl leukotriene receptor 1 partially mediates brain cryoinjury in mice 1
Author(s) -
DING Qian,
FANG Sanhua,
ZHANG Lihui,
ZHANG Weiping,
CHEN Zhong,
WEI Erqing
Publication year - 2007
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2007.00576.x
Subject(s) - receptor , antagonist , receptor antagonist , downregulation and upregulation , chemistry , microglia , pharmacology , medicine , inflammation , biochemistry , gene
Aim : To determine whether the cysteinyl leukotriene receptor 1 (CysLT 1 receptor) modulates brain cryoinjury and whether the CysLT 1 receptor antagonist pranlukast exerts a time‐dependent protective effect on cryoinjury in mice. Methods : Brain cryoinjury was induced by applying a liquid nitrogen‐cooled metal probe to the surface of the skull for 30 s. Brain lesion, neuron density, and endogenous IgG exudation were observed 24 h after cryoinjury. Transcription and the expresion of the CysLT 1 receptor were detected by RT‐PCR and immunoblotting, and the localization of the receptor protein by double immunofluorescence. Results : The mRNA and protein expressions of the CysLT 1 receptor were upregulated in the brain 6‐24 h after cryoinjury, and the CysLT 1 receptor protein was primarily localized in the neurons, not in the neurons, not in the astrocytes or microglia. Pre‐injury treatments with multi‐doses and a single dose of pranlukast (0.1 mg/kg) attenuated cryoinjury, postinjury single dose (0.1 mg/kg) at 30 min (not 1 h) after cryoinjury was also effective. Conclusion : The CysLT 1 receptor modulates cryoinjury in mice at least partly, and postinjury treatment with its antagonist pranlukast exerts the protective effect with a therapeutic window of 30 min.