Open AccessInvolvement of hepatitis B X‐interacting protein (HBXIP) in proliferation regulation of cells 4
Author(s) -
WANG Fengze,
SHA Li,
ZHANG Weiying,
WU Lianying,
QIAO Ling,
LI Nan,
ZHANG Xiaodong,
YE Lihong
Publication year - 2007
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2007.00531.x
Subject(s) - cell growth , microbiology and biotechnology , cell cycle , flow cytometry , biology , transfection , rna interference , cell culture , cell , downregulation and upregulation , western blot , cancer research , gene , rna , biochemistry , genetics
Abstract Aim: To investigat the effect of Hepatitis B X‐interacting protein (HBXIP) on cell proliferation. Methods: A rabbit antibody against HBXIP was generated. The RNA interference (RNAi) fragment of the HBXIP gene was constructed in the pSilencer‐3.0‐H1 vector termed pSilencer‐hbxip. Plasmids of the pcDNA3‐hbxip encoding HBXIP gene and pSilencer‐hbxip were transfected into human breast carcinoma MCF‐7 cells, hepatoma H7402 cells, and the normal human hepatic cell lineL‐O2, respectively. 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazoliumbromide (MTT) assay and 5‐bromo‐2‐deoxyuridine incorporation assay were applied to detect cell proliferation. MCF‐7 cells and L‐O2 cells in the cell cycle were examined by flow cytometry. The proteins involved in cell proliferation and cell cycle were investigated by Western blot. Results: Overexpression of HBXIP resulted in the promotion of proliferation of MCF‐7, H7402, and L‐O2 cells. Flow cytometry showed that the overexpression of HBXIP promoted the cell proliferation of MCF‐7 and L‐O2 cells, and led to an increased cell proliferative index in MCF‐7 cells (from 46.25% to 58.28%) and L‐O2 cells (from 29.62% to 35.54%). Western blot showed that expression levels of c‐Myc, Bcl‐2, and proliferating cell nuclear antigen were upregulated in MCF‐7, H7402, or L‐O2 cells, whereas that of p27 was downregulated. However, the RNAi of HBXIP brought opposite results. Conclusion: One of the functions of HBXIP is its involvement in cell proliferation.