Open AccessResistin‐binding peptide antagonizes role of resistin on white adipose tissue 1
Author(s) -
GU Nan,
HAN Shuping,
FEI Li,
PAN Xiaoqin,
GUO Mei,
CHEN Ronghua,
GUO Xirong
Publication year - 2007
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2007.00480.x
Subject(s) - resistin , adipose tissue , medicine , endocrinology , white adipose tissue , adiponectin , lipid metabolism , chemistry , secretion , adipocyte , biology , obesity , insulin resistance
Aim: To investigate the direct effects of resistin and resistin‐binding peptide (RBP) on lipid metabolism and endocrine function in adipose tissue. Methods : Rat white adipose tissue was cultured in vitro and incubated for 24 h with 30 ng/mL recombinant rat resistin protein (rResistin) or combined with RBP of varying concentrations(1×10 ‐12 mol/L, 1×10 ‐10 mol/L, 1×10 ‐8 mol/L). Free fatty acids (FFA) released into medium was measured by a colorimetric kit. The levels of protein secretion and mRNA expression of TNF‐α and adiponectin were detected by ELISA kit and RT‐PCR respectively. Results : The levels of FFA released into medium were significantly increased after 24 h of exposure to rResistin, but significantly decreased after RBP was applied, although there was no difference between the 3 concentrations. The protein level and gene expression of TNF‐α in adipose tissue were significantly increased after 24 h of exposure to rResistin, but only obviously decreased after incubated with 1×10 ‐8 mol/L RBP. The levels of protein secretion and mRNA expression of adiponectin in adipose tissue were significantly decreased after 24 h of exposure to rResistin, but increased after incubated with RBP with the higher concentrations. Conclusion: RBP can effectively antagonize the role of resistin on the lipid metabolism and endocrine function of adipose tissue.