Open AccessCysteinyl leukotriene receptors CysLT 1 and CysLT 2 are upregulated in acute neuronal injury after focal cerebral ischemia in mice 1
Author(s) -
ZHANG Yanjun,
ZHANG Lei,
YE Yilu,
FANG Sanhua,
ZHOU Yu,
ZHANG Weiping,
LU Yunbi,
WEI Erqing
Publication year - 2006
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2006.00458.x
Subject(s) - downregulation and upregulation , receptor , ischemia , medicine , neuron , brain ischemia , ischemic injury , neuroscience , chemistry , biology , gene , biochemistry , psychiatry
Aim: To determine whether cysteinyl leukotriene receptors (CysLT 1 and CysLT 2 ) are upregulated in acute neuronal injury after focal cerebral ischemia in mice, and to confirm CysLT1 receptor localization. Methods : After permanent focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO), neurological deficits and neuron loss were determined at various time points within 48 h. The mRNA expressions of CysLT 1 and CysLT 2 receptors in the brain were analyzed by RT‐PCR. CysLT1 receptor localization was detected by double immunofluorescence. Results : Neurological deficits and neuron loss were found 6, 24 and 48 h after MCAO. The mRNA expressions of both CysLT1 and CysLT 2 receptors were upregulated in the ischemic hemisphere 1, 24, and 48 h after MCAO with peaks at 24 h. The CysLT 1 receptor was selectively localized in neurons 24 h after MCAO. Conclusion : CysLT 1 and CysLT 2 receptors are upregulated in acute neuronal injury after focal cerebral ischemia, and the CysLT1 receptor is localized in neurons after ischemia.