Open AccessAnti‐inflammatory effects of a novel peptide designed to bind with NF‐κB p50 subunit 1
Author(s) -
WANG Zhenping,
CAI Shaoxi,
LIU Dongbo,
XU Xiang,
LIANG Huaping
Publication year - 2006
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2006.00442.x
Subject(s) - protein subunit , peptide , p50 , nf κb , nfkb1 , chemistry , pharmacology , inflammation , medicine , immunology , biochemistry , transcription factor , gene
Aim: To explore the anti‐inflammatory effects of a novel peptide designed to bind with the NF‐κB p50 subunit. Methods: The affinity of the peptide binding with p50 was measured with a biosensor. Levels of tumor necrosis factor‐α (TNF‐α) and interleukin 6 (IL‐6) from a human acute monocytic leukemia cell line (THP‐1) treated with lipopolysaccharide (LPS) were measured using the ELISA method. In vivo anti‐inflammatory effects of the peptide were tested with phorbol myristate acetate (PMA)‐induced ear edema and zymosan A‐induced peritonitis in mice. Results: The peptide has the ability to interact with the NF‐κB p50 subunit and can effectively inhibit TNF‐α and IL‐6 production in the THP‐1 cell line, PMA‐induced ear edema and zymosan A‐induced peritonitis in mice. Conclusion: The peptide may have therapeutic potential for the treatment of local acute inflammation.