Open AccessCysteinyl leukotriene receptor 1 is involved in N ‐methyl‐ D ‐aspartate‐mediated neuronal injury in mice 1
Author(s) -
DING Qian,
WEI Erqing,
ZHANG Yanjun,
ZHANG Weiping,
CHEN Zhong
Publication year - 2006
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2006.00438.x
Subject(s) - nmda receptor , excitotoxicity , pharmacology , neuroprotection , receptor antagonist , receptor , downregulation and upregulation , glutamate receptor , antagonist , chemistry , medicine , biochemistry , gene
Aim: To determine whether cysteinyl leukotriene receptor 1 (CysLT1 receptor) is involved in N ‐methyl‐ D ‐aspartate (NMDA)‐induced excitotoxic injury in the mouse brain. Methods : Brain injury was induced by NMDA microinjection (50‐150 nmol in 0.5 uL) into the cerebral cortex. The changes in CysLT 1 receptor expression 24 h after NMDA injection and the effects of a CysLT 2 receptor antagonist, pranlukast (0.01 and 0.1 mg/kg), an NMDA receptor antagonist, ketamine (30 mg/kg), and an antioxidant, edaravone (9 mg/kg) were observed. Results : In the NMDA‐injured brain, the CysLT 1 receptor mRNA, and protein expression were upregulated, and the receptor was mainly localized in the neurons and not in the astrocytes. Pranlukast, ketamine and edaravone decreased NMDA‐induced injury; pranlukast (0.1 mg/kg) and ketamine inhibited the upregulated expression of the CysLT1 receptor. Conclusion : CysLT 1 receptor expression in neurons is upregulated after NMDA injection, and NMDA‐induced responses are inhibited by CysLT 1 receptor antagonists, indicating that the increased CysLT1 receptor is involved in NMDA excitotoxicity.