Open AccessDiallyl disulfide suppresses growth of HL‐60 cell through increasing histone acetylation and p21 WAF1 expression in vivo and in vitro 1
Author(s) -
ZHAO Jie,
HUANG Weiguo,
HE Jie,
TAN Hui,
LIAO Qianjin,
SU Qi
Publication year - 2006
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2006.00433.x
Subject(s) - sodium butyrate , acetylation , microbiology and biotechnology , in vivo , cell cycle , flow cytometry , cell growth , diallyl disulfide , in vitro , chemistry , histone , histone h3 , cancer research , biology , apoptosis , cell culture , biochemistry , gene , genetics
Aim: To examine the differentiation induction and growth inhibition of HL‐60 cells by diallyl disulfide (DADS), and its relationship with the alterations of histone acetylation and p21 WAF1 expression in vitro and in vivo . Methods: Differentiation was studied by nitroblue tetrazolium (NBT) reduction of HL‐60 cell in vitro . HL‐60 cells 5×10 6 were injected into the right side of the peritoneal cavity of severe combined immunodeficiency (SCID) mice. When the peritoneal neoplasms were detected, the SCID mice were randomly divided into 3 groups and received an ip injection of vehicle alone (NS), DADS or sodium butyrate (SB). The growth inhibition of peritoneal neoplasms induced by DADS was observed by a growth curve. The cycle distribution of HL‐60 cells in SCID mice was monitored by flow cytometry. The expression of acetylated histone H3, H4 and p21 WAF1 were measured by Western blot. Results: After treatment with DADS for 0–72 h, the NBT reduction ability of HL‐60 cells increased in a time‐dependent manner, compared with no treatment of HL‐60 cells. In the HL‐60 cells treated with DADS for 24 h, the expression of acetylated histone H3, H4, and p21 WAF1 increased obviously. After treatment with DADS, tumor growth was markedly suppressed. HL‐60 cells from mice treated with DADS were blocked in the G 1 phase, from 25.4% to 63.4%. The tumors from the mice treated with DADS showed an increase of acetylated histone H3, H4, and p21 WAF1 . Conclusion: DADS could induce differentiation and inhibit the growth of HL‐60 cells through increasing the expression of acetylated histone H3, H4, and p21 WAF1 in vitro and in vivo .