Open AccessCharacteristics of Ca 2+ ‐exocytosis coupling in isolated mouse pancreatic β cells 1
Author(s) -
GE Qian,
DONG Yongming,
HU Zhitao,
WU Zhengxing,
XU Tao
Publication year - 2006
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2006.00398.x
Subject(s) - exocytosis , beta (programming language) , coupling (piping) , chemistry , microbiology and biotechnology , medicine , biophysics , endocrinology , biology , computer science , biochemistry , secretion , materials science , programming language , metallurgy
Aim: To characterize Ca 2+ ‐stimulated exocytosis in isolated mouse pancreatic β cells. Methods : An improved method was described for isolation of mouse pancreatic β cells by collagenase P. The Ca 2+ channel current and the membrane capacitance were examined by using the whole‐cell patch clamp recording technique. Results : Using depolarization and flash photolysis of caged Ca 2+ to induce Ca 2+ ‐dependent exocytosis in β cell from KM mouse, we have explored the characteristics of the Ca 2+ channel current and the relationship between Ca 2+ signals and exocytosis. The averaged peak Ca 2+ current measured at +20 mV was ‐60±6 pA ( n = 13). Conclusion : We characterized three kinetically different pools of vesicles in mouse pancreatic β cells, namely an immediately releasable pool, a readily releasable pool, and a reserve pool.