Open AccessGinkgo biloba extract suppresses hypertrophy and extracellular matrix accumulation in rat mesangial cells 1
Author(s) -
WANG Jianyun,
YIN Xiaoxing,
WU Yunming,
TANG Daoquan,
GAO Yuanyuan,
WAN Meirong,
HOU Xiaoyu,
ZHANG Bei
Publication year - 2006
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2006.00360.x
Subject(s) - extracellular matrix , mesangial cell , endocrinology , medicine , laminin , muscle hypertrophy , chemistry , glomerulosclerosis , oxidative stress , transforming growth factor , biology , biochemistry , kidney , proteinuria
Aim: To observe the effects of Ginkgo biloba extract (EGb) on the hypertrophy of mesangial cells and the accumulation of extracellular matrix (ECM) in mesangial cells. Methods: Cultured mesangial cells were allotted into 7 groups: normal group, solvent control group, high glucose group, low dose of EGb group, moderate dose of EGb group, high dose of EGb group, and captopril group. Activities of cell antioxidases, S phase percentage and G 0 /G 1 phase percentage, collagen IV and laminin, Smad2/3 and Smad7, TGF‐β 1 mRNA were measured by different methods. Results: For EGb‐treated groups, when compared with high glucose group, the cell percentage of S phase was raised and the percentage of G 0 /G 1 was lowered. The intensity of oxidative stress was weakened. The expression of Smad2/3 was greatly decreased and Smad7 was increased. Collagen IV, laminin and TGF‐β 1 mRNA were also reduced. Conclusion: EGb can suppress cell hypertrophy and the accumulation of ECM in rat mesangial cells, which means it could play a vital role in the delay of glomerulosclerosis in diabetic nephropathy.