Open AccessProtective effects of compound FLZ on β‐amyloid peptide‐(25–35)‐induced mouse hippocampal injury and learning and memory impairment 1
Author(s) -
FANG Fang,
LIU Gengtao
Publication year - 2006
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2006.00347.x
Subject(s) - hippocampus , morris water navigation task , hippocampal formation , chemistry , acetylcholinesterase , medicine , endocrinology , memory impairment , amyloid beta , pharmacology , elevated plus maze , aché , neuroscience , psychology , peptide , biochemistry , enzyme , psychiatry , cognition , anxiety
Aim: To study the protective effects of compound FLZ, a novel synthetic analogue of natural squamosamide, on learning and memory impairment and lesions of the hippocampus caused by icv injection of β‐amyloid 25–35 (Aβ 25–35 ) in mice. Methods : Mice were icv injected with the Aβ 25–35 (15 nmol/mouse), and then treated with oral administration of 75 mg/kg or 150 mg/kg of FLZ once daily for 16 consecutive days. The impairment of learning and memory in mice were tested using step‐down test and Morris water maze test. The content of malondialdehyde (MDA) and the expressions of acetylcholinesterase (AChE), Bax, and Bcl‐2 in the CA1 region of the mouse hippocampus were measured by biochemical and immunohistochemical analysis, respectively. The pathological damages of hippocampus were observed using a microscope. Results : FLZ (75 mg/kg, 150 mg/kg) significantly attenuated Aβ 25–35 ‐induced impairment of learning and memory in the step‐down test and Morris water maze test. FLZ also reduced pathological damages to the hippocampus induced by Aβ 25–35 . Furthermore, FLZ prevented the increase of AChE and Bax, and the decrease of Bcl‐2 immunoreactive cells in the CA1 region of the hippocampus, and reduced the increase of MDA content in the hippocampus in mice injected with Aβ 25–35 . Conclusion : FLZ has protective action against the impairment of learning and memory and pathological damage to the hippocampus induced by icv injection of Aβ 25–35 in mice.