Open AccessEngagement of PSGL‐1 enhances β 2 ‐integrin‐involved adhesion of neutrophils to recombinant ICAM‐1 1
Author(s) -
WANG Xiaoguang,
CHENG Yanping,
BA Xueqing
Publication year - 2006
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2006.00327.x
Subject(s) - selectin , microbiology and biotechnology , integrin , cell adhesion molecule , cell adhesion , cytochalasin d , adhesion , intercellular adhesion molecule 1 , chemistry , intercellular adhesion molecule , e selectin , biology , receptor , cytoskeleton , biochemistry , cell , organic chemistry
Aim: The interactions of selectins and their ligands initiate the process of leukocyte migrating into inflamed tissue. P‐selectin glycoprotein ligand 1 (PSGL‐1) is the best characterized ligand of selectins, and has been demonstrated to mediate the adhesion of leukocytes to all three selectins in vivo. PSGL‐1 not only functions as an anchor molecule to capture the leukocytes to the activated endothelial cells by its interaction with selectins, but also transduces the signals to activate leukocytes. Our present work aimed to investigate the mechanism by which PSGL‐1‐mediated signal activates neutrophils and enhances the adhesion to the endothelial cells. Methods: We detected the effects of the engagement of PSGL‐1 with monoclonal antibodies (mAb) or P‐selectin on the adhesion of neutrophils to the recombinant intercellular adhesion molecule‐1 (ICAM‐1), and on the expression of β 2 ‐integrin. Additionally, the role of cytoskeleton in these process was studied by using inhibitor cytochalasin B. Results: The engagement of PSGL‐1 increased the expression of β 2 ‐integrin on the surface of neutrophils and enhanced the adhesion of neutrophils to the recombinant ICAM‐1. mAb against CD 18 impaired the adhesion of PSGL‐1 ‐engaged neutrophils to ICAM‐1. Moreover, the inhibitor cytochalasin B largely blocked the increase of CD 18 expression as well as the adhesion of PSGL‐1‐engaged neutrophils to ICAM‐1. Conclusion: The PSGL‐1‐transduced signals can enhance β 2 ‐integrin‐involved adhesion of neutrophils to the recombinant ICAM‐1, and this process depends on the dynamics of cytoskeleton.