Open AccessInduction of murine interleukin‐1 beta expression by water‐soluble components from Hericium erinaceum 1
Author(s) -
SON Changgue,
SHIN Jangwoo,
CHO Junghyo,
CHO Chongkwan,
YUN Cheolheui,
HAN Seunghyun
Publication year - 2006
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2006.00321.x
Subject(s) - microbiology and biotechnology , electrophoretic mobility shift assay , chloramphenicol acetyltransferase , gene expression , reporter gene , transcription factor , activator (genetics) , biology , nfkb1 , response element , transcription (linguistics) , messenger rna , promoter , gene , chemistry , biochemistry , linguistics , philosophy
Aim: To investigate the inductive effect of water extract from Hericium erinaceum (WEHE) on interleukin‐1 β (IL‐1β) expression. Methods: A murine macrophage cell‐line, RAW 264.7 was stimulated with 1 to 10 mg/L WEHE and inductions of IL‐1β protein and its steady state mRNA were examined using a bioassay, Western blotting, and reverse transcription‐polymerase chain reaction (RT‐PCR) analysis. The inductive effect of WEHE on IL‐1β gene expression was further investigated by a chloramphenicol acetyltransferase (CAT) reporter gene assay using a transient transfection with pIL‐1(870 bp)‐CAT where the expression of the CAT gene was regulated by a IL‐1β promoter. An electrophoretic mobility shift assay (EMS A) was also performed to examine transcription factors, nuclear factor‐kappa B (NF‐κB), activator protein 1 (AP‐1), nuclear factor interleukin‐6 (NF‐IL6), and cAMP response element (CRE)/activating transcription factor (ATF). Results: WEHE induced IL‐1β production in both its mRNA and protein expression in a dose‐dependent manner. The inductive effect of WEHE on IL‐1β gene expression was due to the augmentation of the IL‐1β transcription. Furthermore, EMSA showed that WEHE markedly increased the binding activities of NF‐κB, and to a lesser extent, those of AP‐1 and NF‐IL6 to their cognate DNA recognition sites, whereas CRE/ATF binding remained constant, all of which are known to be involved in the regulation of IL‐1β gene expression. Conclusion: WEHE induces IL‐1β expression in macrophages at a transcriptional level by enhancing the activation of transcription factors, NF‐κB, NF‐IL6, and AP‐1.