Open AccessRole of 11‐beta‐hydroxysteroid dehydrogenase type 1 in differentiation of 3T3‐L1 cells and in rats with diet‐induced obesity 1
Author(s) -
LIU Yun,
SUN Wenlan,
SUN Yan,
HU Gang,
DING Guoxian
Publication year - 2006
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2006.00316.x
Subject(s) - dehydrogenase , endocrinology , beta (programming language) , medicine , chemistry , biology , microbiology and biotechnology , enzyme , biochemistry , computer science , programming language
Aim: To observe the roles of 11‐beta‐hydroxysteroid dehydrogenase type 1 (11β‐HSD1) in in vitro preadipocyte differentiation and in rats with diet‐induced obesity (DIO). Methods: Protein expression of 11β‐HSD1 in the process of 3T3‐L1 cell differentiation and in various tissues of the rats were detected by Western blot analysis; expression of 11β‐HSD1 mRNA and glucocorticoid receptor (GR) and other marker genes of preadipocyte differentiation were detected by using realtime PCR. Results: Lipid droplets in 3T3‐L1 cells accumulated and increased after stimulation. A dramatically elevated protein level of 11β‐HSD1, especially in the late stages of 3T3‐L1 cell differentiation, was detected. The relative mRNA levels of 11β‐HSD1, GR and cell differentiation markers LPL, aP2, and FAS were upregulated, and Pref‐1 was downregulated during the differentiation. In DIO rats, bodyweight, visceral adipose mass index and the protein expression of 11β‐HSD1 increased, especially in adipose tissue, brain and muscles. Serum insulin, triglyceride, total cholesterol and low‐density lipoprotein cholesterol were found to be increased in DIO rats, but without any obvious changes in blood glucose or tumor necrosis factor‐α levels. Conclusion: 11β‐HSD1 may promote preadipocyte differentiation, and may be involved in the development of obesity.