Open AccessProstaglandin A1 inhibits increases in intracellular calcium concentration, TXA 2 production and platelet activation 1
Author(s) -
ZHU Yi,
GU Zhenlun,
LIANG Zhongqin,
ZHANG Huilin,
QIN Zhenghong
Publication year - 2006
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2006.00311.x
Subject(s) - platelet , chemistry , thrombin , calcium , calcium in biology , platelet activation , prostaglandin , intracellular , adhesion , platelet aggregation , biochemistry , biophysics , medicine , biology , organic chemistry
Aim: In our previous studies we found that cyclopentenane prostaglandin A1 (PGA1) had neuroprotective effects in a rodent ischemic model. In the present study we aimed to investigate the inhibitory effect of PGA 1 on platelet function. Method: The rate of aggregation of human platelets was measured by using turbidimetry. The rate of adhesion of platelets to cultured endothelial cells was determined by using [ 3 H]‐adenine labeled platelets. 5‐Hydroxytryptamine release from platelets was measured with O‐phthaldialdehyde fluorospectrophotometry. The levels of TXB 2 , a stable metabolite of TXA 2 , were determined by radioimmunoassay. Alternations in platelet morphology were observed using an electron microscope, and the intraplatelet free calcium concentrations were measured with Fluo‐3/AM FCM assay. Results: PGA1 significantly inhibited thrombin‐, collagen‐ and ADP‐induced aggregation and adhesion of platelets. The morphological changes of platelets induced by thrombin were blocked by PGA1. PGA1 inhibited the release of 5‐hydroxytyptamine from dense granules and the synthesis of TXA 2 . Conclusion: PGA1 inhibits the activation of platelets probably through blocking increases in intracellular calcium concentration and TXA 2 synthesis.