Open AccessEffects of propyl gallate on interaction between TNF‐α and sTNFR‐I using an affinity biosensor 1
Author(s) -
LI Jing,
HUANG Jiadong,
WU Baoyan,
CHEN Qiang
Publication year - 2005
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2005.00198.x
Subject(s) - methyl gallate , biosensor , gallate , tumor necrosis factor alpha , chemistry , propyl gallate , cuvette , in vivo , stereochemistry , chromatography , biochemistry , nuclear chemistry , biology , endocrinology , gallic acid , physics , microbiology and biotechnology , quantum mechanics , antioxidant
Aim: To study the effects of propyl gallate on the interaction of tumor necrosis factor‐α (TNF‐α) with its soluble receptor, sTNFR‐I. Methods: Interactions between TNF‐α and sTNFR‐I were analyzed using an IAsys biosensor. sTNFR‐I was immobilized on the carboxymethyl dextran (CMD) surface of the IAsys biosensor cuvettes, and TNF‐α preincubated with different concentrations of propyl gallate was added to the cuvettes. The resonant angle shift caused by the binding between TNF‐α and sTNFR‐I was then recorded. Results: sTNFR‐I was immobilized on the CMD surface at a density of 2.76 ng/mm 2 . TNF‐α then bound the immobilized sTNFR‐I specifically, and propyl gallate was able to enhance the binding between TNF‐α and sTNFR‐I in a dose‐dependent manner. Conclusion: The binding between TNF‐α and sTNFR‐I is one of the targets that propyl gallate can act on in vivo. The IAsys biosensor offers a new clue as to the study on the mechanisms of action of propyl gallate.