Open AccessInfluence of dosage forms on pharmacokinetics of daidzein and its main metabolite daidzein‐7‐ O ‐glucuronide in rats 1
Author(s) -
QIU Feng,
CHEN Xiaoyan,
SONG Bo,
ZHONG Dafang,
LIU Changxiao
Publication year - 2005
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2005.00187.x
Subject(s) - daidzein , metabolite , pharmacokinetics , pharmacology , glucuronide , chemistry , active metabolite , medicine , genistein , biochemistry
Aim: To investigate the influence of dosage forms on the pharmacokinetics of daidzein and its main metabolite daidzein‐7‐ O ‐glucuronide in Wistar rats. Methods: After administration of two typical dosage forms (daidzein solution and suspension), the concentrations of daidzein and daidzein‐7‐ O ‐glucuronide were determined by an LC‐MS‐MS method. The pharmacokinetic parameters were calculated and analyzed statistically using the Student's t ‐test. Results: Absorption of daidzein after administration of daidzein solution ( t max = 0.46 h) was more rapid than that of the suspension ( t max = 5.00 h). The peak plasma concentrations of daidzein after administration of daidzein solution and suspension were 601.1 μg/L and 127.3 μg/L, respectively, and those of daidzein‐7‐ O ‐glucuronide were 3000 μg/L and 192.6 μg/L, respectively. The absolute bioavailabilities of free daidzein in rats after administration of daidzein solution and suspension were 12.8% and 6.1%, respectively, which were calculated to be 47.0% and 12.2%, respectively, in the form of total daidzein (free plus conjugated daidzein). Conclusion: Absorption of daidzein solution was better than absorption of suspension ( P <0.05).