Open AccessEffect of daidzin, genistin, and glycitin on osteogenic and adipogenic differentiation of bone marrow stromal cells and adipocytic transdifferentiation of osteoblasts
Author(s) -
LI Xianghui,
ZHANG Jinchao,
SUI Senfang,
YANG Mengsu
Publication year - 2005
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2005.00161.x
Subject(s) - genistin , daidzin , transdifferentiation , adipogenesis , osteoblast , chemistry , biology , endocrinology , adipose tissue , biochemistry , in vitro , genistein , daidzein
Aim: To examine the effect of daidzin, genistin, and glycitin on the osteogenic and adipogenic differentiation of bone marrow stromal cells (MSC) and the adipogenic transdifferentiation of osteoblasts. Methods: MTT test, alkaline phosphatase (ALP) activity measurement, Oil Red O stain and measurement were employed. Results: Daidzin, genistin, and glycitin 1×10 ‐8 , 5×10 ‐7 , 1×10 ‐6 , 5×10 ‐6 , and 1×10 ‐5 mol/L all promoted the proliferation of primary mouse bone MSC and osteoblasts. Daidzin 5×10 ‐7 mol/L and genistin 1×10 ‐6 mol/L promoted the osteogenesis of MSC. Genistin 1×10 ‐8 , 5×10 ‐7 , 1×10 ‐6 , 5×10 ‐6 , and 1×10 ‐5 mol/L and glycitin 1×10 ‐8 , 1×10 ‐6 , and 1×10 ‐5 mol/L inhibited the adipogenesis of MSC. Daidzin, genistin, and glycitin 1×10 ‐8 , 5×10 ‐7 , 1×10 ‐6 , 5×10 ‐6 , and 1×10 ‐5 mol/L all inhibited the adipocytic transdifferentiation of osteoblasts. Conclusions: Daidzin, genistin, and glycitin may modulate differentiation of MSC to cause a lineage shift toward the osteoblast and away from the adipocytes, and could inhibit adipocytic transdifferentiation of osteoblasts. They could also be helpful in preventing the development of osteonecrosis.