Open AccessEffects of navelbine and docetaxel on gene expression in lung cancer cell strains 1
Author(s) -
CAI Li,
DONG Haiying,
SUI Guangjie
Publication year - 2005
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2005.00142.x
Subject(s) - docetaxel , lung cancer , gene , biology , cancer research , gene expression , complementary dna , cancer , oncology , medicine , genetics
Aim: To search genes sensitivity to the anti‐cancer drugs navelbine (NVB) and docetaxel (DOC) in small‐cell lung cancer (SCLC) and non‐small‐cell lung cancer (NSCLC) cell strains. Methods: The sensitivity of 4 strains of SCLC and 6 strains of NSCLC to NVB and DOC was evaluated using the MTT assay. The expression of 1291 sensitive‐related genes to the anti‐cancer drugs in 10 lung cancer cell strains was measured using cDNA macroarrays and the relationship was analyzed. Results: In total, there were 56 (r0.4) genes sensitive to NVB and DOC. For NVB: 36 genes were sensitive to NVB, 20 co‐expressed genes between the SCLC+NSCLC set and the NSCLC set; 27 expressed genes and 7 specially expressed genes in the SCLC+NSCLC set; and 29 expressed genes and 9 specially expressed genes in the NSCLC set. For DOC, 50 genes were sensitive to DOC, 12 co‐expressed genes between the SCLC+NSCLC set and the NSCLC set; 24 expressed genes and 12 specially expressed genes in the SCLC+NSCLC set; and 38 expressed genes and 26 specially expressed genes in the NSCLC set. The genes sensitive to NVB and DOC in lung‐cancer cell stains were mainly divided into the following 4 categories: signal transduction molecules, cell factors, transcription factors and metabolism‐related enzymes and inhibitors. Conclusions: There were obvious differences in genes related to NVB and DOC between SCLC and NSCLC cell strains, but the same as categories of function.