Open AccessPuerarin reduces increased c‐fos, c‐jun, and type IV collagen expression caused by high glucose in glomerular mesangial cells
Author(s) -
MAO Caiping,
GU Zhenlun
Publication year - 2005
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2005.00133.x
Subject(s) - puerarin , protein kinase c , diabetic nephropathy , extracellular matrix , c jun , mesangial cell , c fos , endocrinology , medicine , flow cytometry , junb , extracellular , chemistry , type i collagen , gene expression , kinase , microbiology and biotechnology , kidney , biology , biochemistry , gene , transcription factor , alternative medicine , pathology
Aim: Increased expression of c‐fos, c‐jun and type IV collagen (CoIV) in glomerular mesangial cells (GMC) are important characteristics of diabetic nephropathy. Both c‐fos and c‐jun regulate the gene expression of extracellular matrix components, and CoIV is the main component of the extracellular matrix. It has been reported that puerarin inhibits aggregation of the extracellular matrix in diabetic rats by an as yet unknown mechanism. The aim of this study is to investigate the effect of puerarin on c‐fos, c‐jun and CoIV expression in GMC cultured in medium containing 5.6 or 27.8 mmol/L glucose. Methods: The expressions of c‐fos and c‐jun were measured at the protein level using flow cytometry. CoIV content was detected using radioimmunoassay. Protein kinase C (PKC) activity was measured using liquid scintillation counting. Results: Puerarin (10 −5 mmol/L) significantly ameliorated the high‐glucose effect on c‐fos, c‐jun and CoIV expression. This effect is accompanied by a reduced PKC activity in these cells. Conclusion: Our results suggest that reduced PKC activity and expression of c‐fos and c‐jun in GMC might participate in the mechanisms underlying the therapeutic effect of puerarin on diabetic nephropathy.