Open AccessExpression of pro‐inflammatory and anti‐inflammatory cytokines in brain of atherosclerotic rats and effects of Ginkgo biloba extract 1
Author(s) -
JIAO Yabin,
RUI Yaocheng,
LI Tiejun,
YANG Pengyuan,
QIU Yan
Publication year - 2005
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2005.00106.x
Subject(s) - ginkgo biloba , proinflammatory cytokine , messenger rna , endocrinology , tumor necrosis factor alpha , medicine , blot , downregulation and upregulation , chemistry , inflammation , interleukin , pharmacology , cytokine , biochemistry , gene
Aim: To study the protein and mRNA expressions of pro‐inflammatory and antiinflammatory cytokines in the brain of rats with atherosclerosis (AS) and the effects of Ginkgo biloba extract ( Gb E) on expressions of cytokines. Methods: The experimental model of AS in rats were established by intraperitioneal injection of vitamin D3 with high fat/cholesterol diet. Gb E 100 mg/kg was administered to rats by ig. After 8 weeks, the expressions of IL‐1β, TNF‐α, IL‐10, and IL‐10R in the brain tissues of AS rats were detected by enzyme‐linked immunosorbant assay, immunohistochemistry, Western blotting, and reverse transcriptase polymerase chain reaction. Results: The protein and mRNA expressions of IL‐1β, TNF‐α, and IL‐10 in the brains were markedly higher in AS groups than that in control groups (6.11±0.15, 1.55±0.14, 0.54±0.04 ng/g wet weight vs 0.80±0.14, 0.33±0.09, and 0.33±0.02 ng/g wet weight, respectively). The protein and mRNA expressions of IL‐1β and TNF‐α in the brains were markedly lower in Gb E groups (3.82±0.54, 0.95±0.08 ng/g wet weight) than that in AS groups, the protein and mRNA expressions of IL‐10 and IL‐10R in the brains were markedly higher in Gb E groups (0.85±0.06 ng/g wet weight) than that in AS groups. Conclusion:Gb E inhibited production of pro‐inflammatory cytokines IL‐1β and TNF‐α, but upregulated the production of anti‐inflammatory cytokines, IL‐10 and IL‐10R in brain, which might be related with its anti‐AS actions.