Open AccessTriptolide suppresses CD80 and CD86 expressions and IL‐12 production in THP‐1 cells 1
Author(s) -
LIU Jing,
WU Qingli,
FENG Yonghong,
YANG Yifu,
LI Xiaoyu,
ZUO Jianping
Publication year - 2005
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2005.00035.x
Subject(s) - triptolide , cd86 , thp1 cell line , cd80 , tripterygium wilfordii , chemistry , lipopolysaccharide , in vitro , microbiology and biotechnology , pharmacology , t cell , immunology , cell culture , cd40 , biochemistry , immune system , medicine , biology , cytotoxic t cell , apoptosis , alternative medicine , pathology , genetics
Aim: To investigate the effects of triptolide, a diterpenoid triepoxide from Tripterygium wilfordii Hook F (TWHF), on the co‐stimulatory molecule expression and interleukin‐12 (IL‐12) production from THP‐1 cells. Methods: THP‐1 cells were differentiated into macrophage‐like cells by Me 2 SO, and then cultured with IFN‐γ (500 kU/L) and lipopolysaccharide (LPS) (1 mg/L) with or without triptolide. The surface molecule expressions were analyzed on a FACScan flow cytometer. IL‐12p40, IL‐12p70 were assayed by ELISA. Results: Tripolide suppressed CD80 and CD86 expressions on IFN‐γ (500 kU/L) and LPS (1 mg/L) activated THP‐1 cells at nontoxic dosages of 2.5–0.625 μg/L. Furthermore, the production of IL‐12p40 and IL‐12p70 were also significantly reduced in THP‐1 cells exposed to triptolide. Conclusion: Triptolide impairs the antigen‐presenting function by inhibiting CD80 and CD86 expressions and decreased IL‐12p40 and IL‐12p70 (bioactive form) productions from the activated THP‐1 cells.