Open AccessAntiapoptotic effect both in vivo and in vitro of A20 gene when transfected into rat hippocampal neurons
Author(s) -
MIAO Hongsheng,
YU Luyang,
HUI Guozhen,
GUO Lihe
Publication year - 2005
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2005.00002.x
Subject(s) - hippocampal formation , transfection , in vivo , electroporation , apoptosis , penumbra , in vitro , neuron , transplantation , neuroscience , biology , microbiology and biotechnology , ischemia , medicine , gene , genetics
Aim: To evaluate the antiapoptotic effect of the A20 gene in primary hippocampal neurons both in vivo and in vitro. Methods: Primary hippocampal neurons in embryonic day 18 (E18) rats were transfected with the A20 gene by using the new Nucleofector electroporation transfection method. We then examined, whether A20 ‐neurons possessed anti‐apoptotic abilities after TNF‐α stimulation in vitro. A20‐neurons and pcDNA3 ‐neurons were transplanted into the penumbra of the brains of rats that had been subjected to 90‐min of ischemia induced by left middle cerebral artery occlusion (MCAO). Results: A20‐neurons resisted TNF‐α induced apoptosis in vitro. The apoptosis rate of neurons overexpressing A20 (28.46%±3.87%) was lower than that in neurons transfected with pcDNA3 (53.06%±5.36%). More A20‐neurons survived in the penumbra both 3‐d and 7‐d after transplantation than did sham pcDNA3 neurons. Conclusion: The novel function of A20 may make it a potential targets for the gene therapy for neurological diseases.