Open AccessChemical gene synthesis: strategies, softwares, error corrections, and applications
Author(s) -
Xiong AiSheng,
Peng RiHe,
Zhuang Jing,
Gao Feng,
Li Yi,
Cheng ZongMing,
Yao QuanHong
Publication year - 2008
Publication title -
fems microbiology reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.91
H-Index - 212
eISSN - 1574-6976
pISSN - 0168-6445
DOI - 10.1111/j.1574-6976.2008.00109.x
Subject(s) - computational biology , biology , gene , synthetic biology , genomics , proteomics , dna , dna synthesis , function (biology) , genetics , genome
Chemical synthesis of DNA sequences provides a powerful tool for modifying genes and for studying gene structure, expression and function. Modified genes and consequently protein/enzymes can bridge genomics and proteomics research or facilitate commercial applications of gene and protein technologies. In this review, we will summarize various strategies, designing softwares and error correction methods for chemical gene synthesis, particularly for the synthesis and assembly of long DNA molecules based on polymerase cycling assembly. Also, we will briefly discuss some of the major applications of chemical synthesis of DNA sequences in basic research and applied areas.