Open AccessTernary complex formation on leaderless phage mRNA
Author(s) -
Resch Armin,
Tedin Karsten,
Graschopf Anton,
HaggårdLjungquist Elisabeth,
Bläsi Udo
Publication year - 1995
Publication title -
fems microbiology reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.91
H-Index - 212
eISSN - 1574-6976
pISSN - 0168-6445
DOI - 10.1111/j.1574-6976.1995.tb00197.x
Subject(s) - biology , primer extension , microbiology and biotechnology , messenger rna , start codon , lambda phage , gene , coding region , lac operon , bacteriophage , ribosomal rna , genetics , gene expression , escherichia coli
The phage Lambda p RM promoter‐derived cI mRNA and phage P2 gene V mRNA are transcribed beginning with the A residue of the AUG start codon. Using lacZ fusion analysis we have assessed the effects of alterations in the immediate downstream coding region on the translational efficiency of these mRNAs. Mutations, including deletions of the putative downstream box of either cI or gene V mRNAs, showed no significant reduction in expression of the different lacZ fusions. Primer extension inhibition analysis suggests a role of ribosomal protein S1 in cI mRNA recognition.