Open AccessPeptide presentation by bacteriophage P4
Author(s) -
Lindqvist Bjorn H.,
Naderi Soheil
Publication year - 1995
Publication title -
fems microbiology reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.91
H-Index - 212
eISSN - 1574-6976
pISSN - 0168-6445
DOI - 10.1111/j.1574-6976.1995.tb00185.x
Subject(s) - capsid , bacteriophage , peptide , biology , peptide sequence , amino acid , phage display , oligonucleotide , microbiology and biotechnology , biochemistry , gene , escherichia coli
This article focuses on bacteriophage P4 as a potential peptide display phage by exploring the possibility of using the P4 capsid decoration component, Psu, as a peptide carrier protein. Psu is non‐essential for P4 growth but it enhances the stability of the P4 capsid by binding to its exterior. We have constructed a unique Sac I cloning site in the beginning of the psu gene. This site changes the third amino acid of Psu from Ser to Leu. This substitution does not destroy the binding of Psu to the P4 capsid. A synthetic oligonucleotide encoding a 10‐amino acid peptide whose sequence is part of the human p62 c‐myc protein, has been inserted into the Sac I site. The Psu c‐myc shows full capsid binding activity and reacts with monoclonal antibodies directed against the c‐myc peptide. These results pave the way for the further development of a peptide display system based on bacteriophage p4.