Open AccessPerfusion systems for hybridoma cells based on sedimentation in chambers and erlenmeyer flasks
Author(s) -
Mette Karen,
Lassen Krieger,
Emborg Claus
Publication year - 1994
Publication title -
fems microbiology reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.91
H-Index - 212
eISSN - 1574-6976
pISSN - 0168-6445
DOI - 10.1111/j.1574-6976.1994.tb00078.x
Subject(s) - erlenmeyer flask , laboratory flask , sedimentation , fermentation , biology , perfusion , chromatography , petri dish , microbiology and biotechnology , food science , chemistry , sediment , medicine , paleontology , cardiology
An inclined sedimentation chamber and a modified 250‐ml Erlenmeyer flask have been used as separation devices for perfusion fermentations with hybridoma cells. The maximum cell density is increased 2–16‐fold compared to batch fermentations when the separation units are used. When the sedimentation chamber is used, IgG is continuously produced and the daily production is increased by a factor 3.7 compared to batch fermentation.