Open AccessImprovement of plasmid stability of recombinant Bacillus‐subtilis cells in continuous immobilized cultures
Author(s) -
Castet JoséCharles,
Craynest Muriel,
Barbotin JeanNoël,
Truffaut Nicole
Publication year - 1994
Publication title -
fems microbiology reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.91
H-Index - 212
eISSN - 1574-6976
pISSN - 0168-6445
DOI - 10.1111/j.1574-6976.1994.tb00074.x
Subject(s) - bacillus subtilis , plasmid , recombinant dna , biology , bacillaceae , bacillales , microbiology and biotechnology , bacteria , chromatography , biochemistry , chemistry , dna , gene , genetics
The immobilization of recombinant Bacillus subtilis in K‐carrageenan gel beads has been performed in order to study the growth conditions inside the gel beads and to improve plasmid stability. Bacterial colonies showing high cell density were studied using scanning electron microscopy. A series of continuous cultures of free and immobilized B. subtilis MT119 (pHV1431, pIL252 and pIL252 Kpn) have been developed without selection pressure. In the free‐cell systems, it was found that a loss of plasmid vectors occurred after a short period. In contrast, in the immobilized cell systems, plasmid‐free segregants were not detected in any of the cases during the first 80 h of the culture.