Open AccessIn silico analysis of trypanosomatids' helicases
Author(s) -
Gargantini Pablo R.,
Lujan Hugo D.,
Pereira Claudio A.
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2012.02644.x
Subject(s) - in silico , computational biology , biology , helicase , genetics , evolutionary biology , gene , rna
Trypanosomatids are unicellular protozoan parasites that cause many diseases in animals, including humans, and plants. These early divergent eukaryotes have many singular structures and processes, including the hyper‐modified ‘base J’, a mitochondrial DNA network, RNA editing, and trans‐splicing; all of these unique features involve a wide variety of specific DNA / RNA helicases. In this work, the genomes of trypanosomatids were analyzed by data mining, searching for genes coding for DNA / RNA helicases. Specific motifs and full‐length sequences from all families present in the helicase's superfamilies ( SF s) 1 and 2 were used as baits for genome analyses. A total of 328 putative helicases were identified; 204 genes were assigned to the SF 2, 42 genes to the SF 1, and 76 genes remain unclassified. Eight species‐specific SF 2 helicases were also found; T rypanosoma cruzi has three DEAD ‐box and one DEAH / RHA ‐specific helicases, while L eishmania major has three S wi2/ S nf2 and T rypanosoma brucei has only one R ig I helicase. Finally, to identify helicases that could be used as future therapeutic targets, all obtained genes were compared with those present in the human genome. Forty‐two helicases underrepresented in the human genome were identified; constituting 16 orthologs groups from L . major , T . brucei , and T . cruzi .