Open AccessAn untapped bacterial cellulolytic community enriched from coastal marine sediment under anaerobic and thermophilic conditions
Author(s) -
Ji Shiqi,
Wang Shian,
Tan Yang,
Chen Xiaohua,
Schwarz Wolfgang,
Li Fuli
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2012.02636.x
Subject(s) - clostridium thermocellum , cellulase , fermentation , thermophile , food science , biology , cellulose , butyric acid , microbial consortium , clostridia , clostridium , microbiology and biotechnology , 16s ribosomal rna , biochemistry , bacteria , gene , microorganism , enzyme , genetics
A bacterial community with strong cellulose [filter paper (FP) and microcrystalline cellulose] degradation ability was isolated from the coastal marine environment. They were isolated under thermophilic (60 °C) and anaerobic cultivation conditions. The library of 16 S r RNA gene clones revealed a total of 16 operational taxonomic units after 50 clones were surveyed. Sixty percent of the clones were most related to the type strain of C lostridium thermocellum with 16 S r RNA gene identity around 87–89%. All of them showed extremely low sequence similarities and were novel at least in species level. The gene clone libraries of glycosyl hydrolase family 48 showed low gene and amino acid sequence similarities around 70–72%. The results indicated that the cellulose degradation systems in the specific environment have not been well studied. The enrichment could disrupt FP within 3 days in a basal medium. The cellulase activity of the community was comparable to that of C . thermocellum LQR 1. The main fermentation products were ethanol, acetic acid and butyric acid. This work identified a novel microbial resource with a potential in lignocellulose conversion and biofuel production.