Open AccessAn efficient and reproducible method for transformation of genetically recalcitrant bifidobacteria
Author(s) -
Serafini Fausta,
Turroni Francesca,
Guglielmetti Simone,
Gioiosa Laura,
Foroni Elena,
Sanghez Valentina,
Bartolomucci Alessandro,
Motherway Mary O'Connell,
Palanza Paola,
Sinderen Douwe,
Ventura Marco
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2012.02605.x
Subject(s) - bifidobacterium bifidum , electroporation , transformation (genetics) , plasmid , microbiology and biotechnology , bifidobacterium , biology , chloramphenicol , bifidobacterium longum , dna , computational biology , antibiotics , bacteria , genetics , lactobacillus , gene
This study describes an efficient transformation system for the introduction of plasmid DNA into B ifidobacterium bifidum PRL 2010 and B ifidobacterium asteroides PRL 2011, for which to the best of our knowledge no transformation data have been reported previously. The method is based on electroporation of bifidobacterial cells, which were made competent by an optimized methodology based on varying media and growth conditions. Furthermore, the transformation protocol was applied in order to design a PRL 2010‐derivative, which carries antibiotic resistance against chloramphenicol and which was used to monitor PRL 2010 colonization in a murine model.