Open AccessCyclic‐β‐glucans of R hizobium ( S inorhizobium ) sp. strain NGR 234 are required for hypo‐osmotic adaptation, motility, and efficient symbiosis with host plants
Author(s) -
GayFraret Jérémie,
Ardissone Silvia,
Kambara Kumiko,
Broughton William J.,
Deakin William J.,
Quéré Antoine
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2012.02595.x
Subject(s) - biology , sinorhizobium meliloti , rhizobium , microbiology and biotechnology , vigna , sinorhizobium , bacteria , periplasmic space , mutant , strain (injury) , rhizobiaceae , symbiosis , gene , biochemistry , escherichia coli , genetics , botany , anatomy
Cyclic‐β‐glucans ( C β G ) consist of cyclic homo‐polymers of glucose that are present in the periplasmic space of many G ram‐negative bacteria. A number of studies have demonstrated their importance for bacterial infection of plant and animal cells. In this study, a mutant of R hizobium ( S inorhizobium ) sp. strain NGR 234 ( NGR 234) was generated in the cyclic glucan synthase ( ndvB )‐encoding gene. The great majority of C β G produced by wild‐type NGR 234 are negatively charged and substituted. The ndvB mutation abolished C β G biosynthesis. We found that, in NGR 234, a functional ndvB gene is essential for hypo‐osmotic adaptation and swimming, attachment to the roots, and efficient infection of V igna unguiculata and L eucaena leucocephala .