Open AccessIsolation of new P seudomonas tolaasii bacteriophages and genomic investigation of the lytic phage BF 7
Author(s) -
SajbenNagy Enikő,
Maróti Gergely,
Kredics László,
Horváth Balázs,
Párducz Árpád,
Vágvölgyi Csaba,
Manczinger László
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2012.02592.x
Subject(s) - podoviridae , lytic cycle , biology , bacteriophage , genome , microbiology and biotechnology , siphoviridae , genetics , gene , virus , escherichia coli
Sixteen lytic bacteriophages that infect P seudomonas tolaasii LMG 2342 T were isolated from smashed sporocarps of oyster mushroom ( P leurotus ostreatus ) showing necrotic symptoms. On the basis of the host range investigation of the phages, they have wide infection abilities against the genus P seudomonas , mainly in the case of phages B f3, B f7, B f10, and B f15. Molecular investigations have revealed that they all have ds DNA genomes about 40 kbp in size. Identical restriction patterns resulting from restriction enzyme analysis suggest that the isolates probably belong to the same phage species. However, there was a difference between these phage isolates in their infecting abilities. Phage isolate B f7 was investigated and characterized more deeply. Morphological characterization of B f7 by transmission electron microscopy ( TEM ) has shown that it has a short, noncontractile tail, an icosahedral phage head, and the size is about 60 nm in diameter, suggesting that it belongs to the P odoviridae family. Complete genome sequence analysis of the B f7 phage isolate revealed a 40 058 bp genome, 58.4% G + C content, 46 open reading frames encoding different proteins showing homology to proteins of the bacteriophage C aulobacter crescentus φCd1 from the P odoviridae family. On the basis of these results and comparative genomic studies, we classified the B f7 phage to the subfamily of A utographivirinae , φ KMV ‐like phages.