Open AccessGenomic sequence‐based discovery of novel angucyclinone antibiotics from marine S treptomyces sp. W 007
Author(s) -
Zhang Hongyu,
Wang Hongbo,
Wang Yipeng,
Cui Hongli,
Xie Zeping,
Pu Yang,
Pei Shiqian,
Li Fuchao,
Qin Song
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2012.02582.x
Subject(s) - polyketide , streptomyces , biology , gene cluster , secondary metabolite , polyketide synthase , secondary metabolism , gene , microbiology and biotechnology , bacteria , genetics , biosynthesis
A large number of novel bioactive compounds were discovered from microbial secondary metabolites based on the traditional bioactivity screenings. Recent fermentation studies indicated that the crude extract of marine S treptomyces sp. W007 possessed great potential in agricultural fungal disease control against P homopsis asparagi, P olystigma deformans, C ladosporium cucumerinum, M onilinia fructicola, and C olletotrichum lagenarium . To further evaluate the biosynthetic potential of secondary metabolites, we sequenced the genome of S treptomyces sp. W007 and analyzed the identifiable secondary metabolite gene clusters. Moreover, one gene cluster with type II PKS implied the possibility of S treptomyces sp. W 007 to produce aromatic polyketide of angucyclinone antibiotics. Therefore, two novel compounds, 3‐hydroxy‐1‐keto‐3‐methyl‐8‐methoxy‐1,2,3,4‐tetrahydro‐benz[α]anthracene and kiamycin with potent cytotoxicities against human cancer cell lines, were isolated from the culture broth of S treptomyces sp. W 007. In addition, other four known angucyclinone antibiotics were obtained. The gene cluster for these angucyclinone antibiotics could be assigned to 20 genes. This work provides powerful evidence for the interplay between genomic analysis and traditional natural product isolation research.