Open AccessMechanisms of protein kinase S ch9 regulating B cy1 in S accharomyces cerevisiae
Author(s) -
Zhang Aili,
Gao Wenxuan
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2012.02552.x
Subject(s) - saccharomyces cerevisiae , microbiology and biotechnology , protein kinase a , cytoplasm , phosphorylation , chemistry , biology , biochemistry , yeast
In this study, we investigated the mechanisms of S ch9 regulating the localization and phosphorylation of B cy1. Our research indicated that S ch9 regulated localization of B cy1 via Z ds1 for the following reasons: (1) deletions of SCH9 or ZDS1 both caused nuclear localization of B cy1; (2) S ch9 and Z ds1 interacted physically; (3) overexpression of ZDS1 led to a significantly increased cytoplasmic localization of B cy1 in sch9Δ cells, whereas overexpression of SCH9 had no visible effect on cytoplasmic localization of B cy1 in zds1Δ cells. Our study also suggested that S ch9 regulated phosphorylation of B cy1 via Y ak1.