Open AccessK lebsiella pneumoniae secretes outer membrane vesicles that induce the innate immune response
Author(s) -
Lee Je Chul,
Lee Eun Jeoung,
Lee Jung Hwa,
Jun So Hyun,
Choi Chi Won,
Kim Seung Il,
Kang Sang Sun,
Hyun Sunghee
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2012.02549.x
Subject(s) - klebsiella pneumoniae , microbiology and biotechnology , innate immune system , biology , bacterial outer membrane , immune system , pathogen , effector , proinflammatory cytokine , immunology , inflammation , escherichia coli , gene , biochemistry
Outer membrane vesicles ( OMV s) derived from pathogenic G ram‐negative bacteria are an important vehicle for delivery of effector molecules to host cells, but the production of OMV s from K lebsiella pneumoniae , an opportunistic pathogen of both nosocomial and community‐acquired infections, and their role in bacterial pathogenesis have not yet been determined. In the present study, we examined the production of OMV s from K . pneumoniae and determined the induction of the innate immune response against K . pneumoniae OMV s. Klebsiella pneumoniae ATCC 13883 produced and secreted OMV s during in vitro culture. Proteomic analysis revealed that 159 different proteins were associated with K . pneumoniae OMV s. K lebsiella pneumoniae OMV s did not inhibit cell growth or induce cell death. However, these vesicles induced expression of proinflammatory cytokine genes such as interleukin ( IL )‐1β and IL ‐8 in epithelial cells. An intratracheal challenge of K . pneumoniae OMV s in neutropenic mice resulted in severe lung pathology similar to K . pneumoniae infection. In conclusion, K . pneumoniae produces OMV s like other pathogenic G ram‐negative bacteria and K . pneumoniae OMV s are a molecular complex that induces the innate immune response.