Open AccessBacterial adhesion, intracellular survival and cytokine induction upon stimulation of mononuclear cells with planktonic or biofilm phase S taphylococcus epidermidis
Author(s) -
Spiliopoulou Anastasia I.,
Kolonitsiou Fevronia,
Krevvata Maria I.,
Leontsinidis Michalis,
Wilkinson Thomas S.,
Mack Dietrich,
Anastassiou Evangelos D.
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2012.02533.x
Subject(s) - biofilm , staphylococcus epidermidis , microbiology and biotechnology , peripheral blood mononuclear cell , monocyte , proinflammatory cytokine , bacteria , cytokine , intracellular , biology , intracellular parasite , staphylococcus aureus , immune system , immunology , chemistry , inflammation , in vitro , biochemistry , genetics
S taphylococcus epidermidis is a leading cause of hospital‐acquired and biofilm‐associated infections. Interactions of peripheral blood mononuclear cells ( PBMCs ) and monocyte‐derived macrophages with planktonic or biofilm phase S . epidermidis cells were studied. Biofilm phase bacteria exhibited higher attachment, as well as, a 10‐fold higher intracellular survival in monocyte‐derived macrophages than their planktonic counterparts. Stimulation of PBMCs and monocyte‐derived macrophages was performed with live or formalin‐fixed bacterial cells. Supernatant concentration of selected cytokines was measured by L uminex ® xMAP ™ technology at different time points. As compared to planktonic phase, biofilm phase bacteria elicited lower amounts of proinflammatory cytokines and T h1 response cytokines, such as TNF α, IL ‐12p40, IL ‐12p70 and IFN ‐γ, whereas they enhanced production of IL ‐8, GM ‐ CSF and IL ‐13. This phenomenon was independent of formalin pretreatment. Taken together, these results may contribute to interpretation of observed silent course of biofilm‐associated infections.