Open AccessGenomic screening for C hlamydophila pneumoniae ‐specific antigens using serum samples from patients with primary infection
Author(s) -
Yasui Yumiko,
Yanatori Izumi,
Kawai Yasuhiro,
Miura Koshiro,
Suminami Yoshinori,
Hirota Tomomitsu,
Tamari Mayumi,
Ouchi Kazunobu,
Kishi Fumio
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2012.02520.x
Subject(s) - chlamydophila pneumoniae , serology , biology , antigen , microbiology and biotechnology , virology , chlamydiaceae , antibody , chlamydiales , pathogen , immunology
C hlamydophila pneumoniae , an obligate intracellular human pathogen, causes respiratory tract infections. The most common techniques used for the serological diagnosis of C . pneumoniae infections are microimmunofluorescence tests and commercial serological ELISA tests; these are based on the detection of antibodies against whole chlamydial elementary bodies and lipopolysaccharide/outer membrane protein, respectively. Identification of more specific and highly immunodominant antigens is essential for the development of new serodiagnostic assays. To identify novel specific antigens from C . pneumoniae , we screened 455 genes with unknown function in the genome of C . pneumoniae J138. Extracts of S accharomyces cerevisiae cells expressing GFP‐tagged C . pneumoniae proteins were subjected to W estern blot analysis using serum samples from C . pneumoniae ‐infected patients as the primary antibodies. From this comprehensive analysis, 58 clones expressing C . pneumoniae open reading frames, including hypothetical proteins, were identified as antigens. These results have provided useful information for the development of new serological tools for the diagnosis for C . pneumoniae infections and for the development of vaccines in future.