Open AccessThe iron‐regulated surface proteins IsdA , IsdB , and IsdH are not required for heme iron utilization in S taphylococcus aureus
Author(s) -
Hurd Alexander F.,
GarciaLara Jorge,
Rauter Yvonne,
Cartron Michaël,
Mohamed Ramlan,
Foster Simon J.
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2012.02502.x
Subject(s) - staphylococcus aureus , mutant , heme , microbiology and biotechnology , chemistry , escherichia coli , bacteria , gene , biology , biochemistry , genetics , enzyme
The iron‐regulated surface determinant proteins ( Isd ) of S taphylococcus aureus are expressed during iron limitation and have been proposed to be involved in the scavenging of iron from heme. In this study, the genes encoding the surface proteins IsdA , IsdB , and IsdH were inactivated in order to determine their combined role. The triple mutant was found to have no defect in growth under any conditions of iron limitation tested. Also using a mouse septic arthritis model of S . aureus systemic disease, no significant difference in bacterial load was observed for the triple mutant, compared with its otherwise isogenic parent.