Open AccessDeletion of yncD gene in S almonella enterica ssp. enterica serovar T yphi leads to attenuation in mouse model
Author(s) -
Xiong Kun,
Chen Zhijin,
Xiang Guiming,
Wang Jing,
Rao Xiancai,
Hu Fuquan,
Cong Yanguang
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2011.02481.x
Subject(s) - salmonella enterica , mutant , virulence , biology , microbiology and biotechnology , gene , plasmid , salmonella typhi , transformation (genetics) , operon , genetics , escherichia coli
TonB‐dependent transporters ( TBDT s) are bacterial outer membrane proteins that are usually involved in the uptake of certain key nutrients, for example iron. In the genome of S almonella enterica ssp. enterica serovar T yphi, the yncD gene encodes a putative TBDT and was identified recently as an in vivo ‐induced antigen. In the present study, a yncD ‐deleted mutant was constructed to evaluate the role of the yncD gene in virulence. Our results showed that the mutant is attenuated in a mouse model by intraperitoneal injection and its virulence is restored by the transformation of a complement plasmid. The competition experiments showed that the survival ability of the yncD ‐deleted mutant decreases significantly in vivo . To evaluate its vaccine potential, the yncD ‐deleted mutant was inoculated intranasally in the mouse model. The findings demonstrated a significant immunoprotection against the lethal wild‐type challenge. The regulation analysis showed that yncD gene promoter is upregulated under acidic condition. The present study demonstrates that the yncD gene plays an important role in bacterial survival inside the host and is suitable for the construction of attenuated vaccine strains as a candidate target gene.